Ideal for the preparation of reagents and for use in enzymatic reactions. For pcr work and plasmid dna storage which water is best to use?
Nuclease free water is used in order to dilute the concentration of the reagents to the proper final.
Nuclease free water in pcr. Can ultra pure water (dnase and rnase free) serve the purpose of nuclease free water?. We must use nuclease free water in pcr reaction so as to prevent degredation of dna. And for pcr, i make 1:5 to 1:25 dilution (in sterile distilled water) of my dna as working templates.
The presence of nucleases such as dnase and rnase in water can degrade precious molecular samples and even ruin experiments. Only if i dilute the dna at least 1:5 in water, do i get a signal. If i used my dna at full concentration, my pcr reactions often do not work.
Even purified water can have a high ph and minerals that can interfere with reactions that require specific salt and ph conditions. Available in 25 ml and 100 ml sizes. Also use of nuclease free water helps avoid dna degradation by nucleases as well as interference of the pcr reaction by ions which could be present in otherwise not nuclease free deionized water.
Ambion offers three different grades of high quality water: No toxic agents, such as depc, are used in production, so as to avoid inhibition in enzymatic reactions. Nuclease free water is used in order to dilute the concentration of the reagents to the proper final concentration.
Each lot tested for nuclease contamination. Ibi pcr grade water is designed specifically for pcr work due to its small volume packaging. As commercially available nuclease free.
Nuclease free water (depc free) has not been treated with depc and is certified for all molecular biology applications requiring nuclease and nucleic acid free water. The 2 ml vial provides convenient use. So, i keep my dna stocks in te.
Pcr, cdna synthesis, or qpcr).